Prokaryotic Protein nthuav qhia
Lub prokaryotic E. coli qhia system yog dav lees paub tias yog ib qho txiaj ntsig zoo, kev paub tab, thiab feem ntau siv cov txheej txheem rau kev qhia cov protein.Ntawm Bioantibody, peb tau cog lus los muab peb cov neeg siv khoom nrog cov kev pabcuam dav dav, ib qho chaw nres tsheb txij li cov noob synthesis mus rau kev qhia cov protein thiab kev huv.Peb cov kev pabcuam suav nrog kev ua kom zoo codon pub dawb thiab siv peb cov cuab yeej cuab tam los daws cov teeb meem ntsig txog kev qhia qis thiab kev tsis sib haum xeeb uas yuav tshwm sim thaum tag nrho cov lus qhia thiab kev huv huv.Peb cov neeg muas zaub tsuas yog yuav tsum tau muab cov noob los yog cov amino acid ib ntus ntawm cov protein thiab peb tuaj yeem xa cov protein zoo nyob rau hauv FAST li peb lub lis piam.Tsis tas li ntawd, Bioantibody muab cov endotoxin tshem tawm thiab tagging cov kev pabcuam raws li peb cov neeg siv khoom xav tau tshwj xeeb.Peb tau cog lus tias yuav xa cov txiaj ntsig thiab cog lus tias yuav tsis them tus nqi yog tias qhov kawg ntawm cov protein tsis qhia.
| Cov khoom siv | Cov ntsiab lus sim | Lub Sijhawm Lead (BD) |
| Gene Synthesis | Codon optimization, noob synthesis thiab subcloning. | 5-10 |
| Kev Qhia Txog Kev Qhia thiab Solubility Analysis | 1. Kev hloov pauv thiab incubation, qhia pom nrog SDS-PAGE.2. Solubility analysis, SDS-PAGE thiab WB detection | 10 |
| Loj incubation thiab purification, kawg protein (purity> 85%, 90%, 95%) thiab cov qauv kev sim | Affinity purification (Ni kem, MBP, GST) |
Yog hais tias cov noob yog synthesized nyob rau hauvBioantibody, lub tsev plasmid yuav suav nrog hauv cov khoom xa tuaj.