Monkeypox Virus Real Time PCR Kit

Lus piav qhia luv luv:

Cat.Tsis muaj	B001P-01	Loj	48 Tests/Kit
Cov qauv	Serum / Lesion Exudate	Trans.& Sto.Temp.	-25 ~ 15 ℃
Hom ntawv	Premix tau lyophilized thiab aliquoted nyob rau hauv 8 strip PCR raj
Siv tau Instrument	Real-time PCR ntsuas xws li ABI7500, Bio-Rad CFX96, SLAN-96S, QuantStudio thiab BTK-8

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Product Detail

Khoom cim npe

Cov ntsiab lus ntawm cov khoom

Npaj siv

Nws yog siv rau kev tshawb pom ntawm Monkeypox Virus hauv tib neeg cov ntshav los yog cov kab mob exudate los ntawm kev siv lub sijhawm PCR systems.

Test Principple

Cov khoom no yog fluorescent sojntsuam raws li Taqman® real-time PCR assay system.Cov primers tshwj xeeb thiab kev sojntsuam yog tsim los rau kev tshawb pom ntawm F3L noob ntawm Monkeypox Virus.Kev tswj sab hauv lub hom phiaj ntawm tib neeg cov noob khaws cia saib xyuas cov qauv sau, coj ua qauv thiab cov txheej txheem PCR tiag tiag kom tsis txhob muaj qhov tsis zoo-tsis zoo.Cov khoom siv yog cov txheej txheem lyophilized tag nrho, uas suav nrog cov ntaub ntawv xav tau rau Monkeypox Virus kuaj pom: nucleic acid amplification enzyme, UDG enzyme, cov tshuaj tiv thaiv tsis zoo, tshwj xeeb primer thiab sojntsuam.

Cov ntsiab lus tseem ceeb

Cheebtsam muab tau teev nyob rau hauv lub rooj.

Cheebtsam	Pob	Cov khoom xyaw
Monkeypox VirusLyophilized Premix	8 strip PCR raj× 6 lub hnab	Primers, probes, dNTP/dUTP Mix, Mg2+, Taq DNA polymerase, UDG Enzyme
MPV Zoo Tswj	400 l x 1 tub	DNA sequences muaj Target gene
MPV Negative Control	400 l x 1 tub	DNA sequences uas muaj tib neeg gene segment
Kev daws teeb meem	1 mL x 1 tub	Stabilizer
Certificate of Conformity	1 qob	/

Kev khiav dej num

1. QauvSau:Cov qauv yuav tsum tau muab sau rau hauv cov raj uas tsis muaj menyuam raws li

nrog cov txheej txheem technical specifications.

2. Kev Npaj Reagent (Reagent Preparation Area)

Tshem tawm cov khoom ntawm cov khoom siv, sib npaug ntawm chav tsev kub rau kev siv standby.

3. Specimen Processing (Specimen Processing Area)

3.1 Nucleic acid extraction

Nws raug nquahu kom coj 200μL cov qauv ua kua, Kev Tswj Xyuas Zoo thiab Kev Tswj Tsis Zoo rau nucleic acid extraction, raws li cov kev xav tau thiab cov txheej txheem ntawm cov kab mob DNA rho tawm cov khoom siv.

3.2 Lyophilized hmoov dissolving thiab template ntxiv

Npaj Monkeypox Virus Lyophilized premix raws li tus naj npawb ntawm cov qauv.Ib qho piv txwv xav tau ib lub raj PCR uas muaj Lyophilized premix hmoov.Kev tswj tsis zoo & kev tswj zoo yuav tsum tau kho raws li ob qho qauv.

(1) Ntxiv 15μL Dissolving Solution rau hauv txhua lub PCR raj uas muaj Lyophilized premix, tom qab ntawd ntxiv 5μL rho tawm cov qauv / Kev Tswj Tsis Zoo / Kev Tswj Zoo rau hauv txhua lub PCR raj raws li.

(2) Npog PCR hlab kom nruj, flick PCR hlab ntawm tes kom txog thaum lyophilized hmoov yog yaj tag thiab sib tov, sau cov kua rau hauv qab ntawm PCR hlab los ntawm instantaneous low-speed centrifugation.

(3) Yog tias siv cov cuab yeej PCR tiag tiag rau kev tshawb nrhiav, ces ncaj qha hloov PCR hlab mus rau thaj chaw amplification;Yog tias siv BTK-8 rau kev kuaj pom, ces yuav tsum tau ua cov haujlwm hauv qab no: hloov 10 μL kua los ntawm PCR raj mus rau cov tshuaj tiv thaiv nti zoo ntawm BTK-8.Ib lub PCR raj sib raug rau ib qho zoo ntawm lub nti.Thaum lub sijhawm ua haujlwm pipette, xyuas kom meej tias lub pipette yog 90 degrees ntsug.Cov lus qhia aerosol barrier pipette yuav tsum muab tso rau hauv nruab nrab ntawm lub qhov dej nrog lub zog nruab nrab thiab tsis txhob thawb lub pipette thaum nws mus txog thawj lub iav (kom tsis txhob muaj npuas).Tom qab lub qhov dej puv lawm, tshem tawm cov tshuaj tiv thaiv daim nyias nyias los npog tag nrho cov qhov dej thiab cov nti raug xa mus rau qhov chaw nrhiav kom pom kev nthuav dav.

4. PCR Amplification (Nrhiav Thaj Chaw)

4.1 Muab cov PCR raj / cov tshuaj tiv thaiv nti rau hauv lub tank tshuaj tiv thaiv thiab teeb tsa cov npe ntawm txhua qhov tshuaj tiv thaiv zoo nyob rau hauv qhov kev txiav txim.

4.2 Cov chaw kuaj pom fluorescence: (1) Monkeypox virus (FAM);(2) Internal Control (CY5).

4.3 Khiav cov txheej txheem caij tsheb kauj vab hauv qab no

Cov txheej txheem ntawm ABI7500, Bio-Rad CFX96, SLAN-96S, QuantStudio:

Cov kauj ruam		Kub	Sijhawm	Lub voj voog
1	Pre-denaturation	95 ℃	2 feeb	1
2	Denaturation	95 ℃	10s ib	45
2	Annealing, extension, fluorescence nrhiav tau	60 ℃	30s ib	45

Txoj Cai ntawm BTK-8:

Cov kauj ruam		Kub	Sijhawm	Lub voj voog
1	Pre-denaturation	95 ℃	2 feeb	1
2	Denaturation	95 ℃	5 s ib	45
2	Annealing, extension, fluorescence nrhiav tau	60 ℃	14s ib	45

5. Kev txheeb xyuas cov txiaj ntsig (thov mus rau Instrument User Manual)

Tom qab cov tshuaj tiv thaiv, cov txiaj ntsig yuav tau txais kev cawmdim.Nyem "Tsim" txhawm rau txheeb xyuas, thiab qhov ntsuas yuav cia li txhais Ct qhov tseem ceeb ntawm txhua tus qauv hauv kab ntawv.Cov kev tswj tsis zoo thiab qhov zoo yuav tsum ua raws li hauv qab no "6. Kev Tswj Xyuas Zoo".

6. Kev Tswj Xyuas Zoo

6.1 Kev Tswj Tsis Zoo: Tsis muaj Ct lossis Ct> 40 hauv FAM channel, Ct≤40 hauv CY5 channel nrog ib txwm ua kom nrawm nrawm.

6.2 Kev Tswj Xyuas Zoo: Ct≤35 hauv FAM channel nrog ib txwm ua kom muaj zog nkhaus, Ct≤40 hauv CY5 channel nrog ib txwm ua kom nthuav dav nkhaus.

6.3 Cov txiaj ntsig tau siv tau yog tias tag nrho cov txheej txheem saum toj no tau ua tiav.Txwv tsis pub, qhov tshwm sim yog invalid.

Kev txhais lus

Cov txiaj ntsig hauv qab no tuaj yeem ua tau:

	Ct tus nqi ntawm FAM channel	Ct tus nqi ntawm CY5 channel	Txhais lus
1#	Tsis muaj Ct lossis Ct> 40	≤ 40	Monkeypox tus kab mob tsis zoo
2#	≤ 40	Tej yam tshwm sim	Monkeypox tus kab mob zoo
3#	40-45	≤ 40	Rov kuaj dua;Yog tias nws tseem yog 40 ~ 45, qhia ua 1 #
4#	Tsis muaj Ct lossis Ct> 40	Tsis muaj Ct lossis Ct> 40	Tsis raug

Nco tseg: Yog tias qhov tshwm sim tsis raug, cov qauv yuav tsum tau sau thiab sim dua.

Cov ntaub ntawv xaj

Khoom npe	Cat.Tsis muaj	Loj	Cov qauv	Txee lub neej	Trans.& Sto.Temp.
Monkeypox Virus Real Time PCR Kit	B001P-01	48 xeem/kit	Serum / Lesion Exudate	12 Lub Hlis	-25 ~ 15 ℃