Kab Cell Protein Expression
Kab kab qhia kab ke yog ib qho kev siv eukaryotic nthuav qhia rau kev nthuav qhia cov protein loj-molecular.Piv nrog rau cov kab mob mammalian, kab kab mob kab mob yog qhov yooj yim thiab tsis tas yuav CO2.Baculovirus yog ib hom kab mob DNA uas muaj kab mob uas muaj kab mob uas muaj kab mob ua kab mob.Nws muaj ntau hom tshwj xeeb, tsis kis kab mob vertebrates, thiab tsis muaj teeb meem rau tib neeg thiab tsiaj txhu.sf9, feem ntau siv los ua tus tswv cell, tshwm hauv planktonic lossis adherent hauv kab lis kev cai.sf9 yog qhov tsim nyog rau kev nthuav dav loj, thiab tuaj yeem siv rau kev ua haujlwm tom ntej thiab hloov kho cov proteins xws li phosphorylation, glycosylation, thiab acylation.Kab kab qhia kab ke kuj tseem siv tau rau kev nthuav qhia ntau lub noob, thiab tuaj yeem nthuav qhia cov tshuaj lom neeg xws li tshuaj tua kab mob peptides.
| Cov khoom siv | Lub Sijhawm Lead (BD) |
| Codon optimization, noob synthesis thiab subcloning | 5-10 |
| P1 tiam kab mob incubation thiab me me qhia | 10-15 |
| P2 tiam kab mob incubation, loj scale qhia thiab purification, xa cov purified protein thiab kev sim qhia |