Monoclonal Antibody Service
Monoclonal antibody, tsim los ntawm ib leeg B cell clone, muaj ntau homogeneity uas tsom rau ib qho antigen epitope.Monoclonal antibody muaj ntau yam zoo, nrog rau siab purity, rhiab heev, thiab tshwj xeeb.Hybridomas yog tsim los ntawm kev sib xyaw ntawm B lymphocytes uas tsim qee cov tshuaj tiv thaiv kab mob, nrog rau cov kab mob myeloma ntev.Bioantibody siv lub tshuab ua haujlwm zoo fusion uas yog 20 npaug zoo dua li cov qauv fusion.Tsis tas li ntawd, nws ntiav cov protein microarray tshuaj ntsuam xyuas tshuab los txheeb xyuas cov tshuaj tiv thaiv kab mob monoclonal uas nthuav tawm qhov tshwj xeeb, kev sib raug zoo, thiab kev ua haujlwm tau zoo tiv thaiv qee qhov epitopes.
Cov khoom siv | Cov ntsiab lus sim | Lub Sijhawm Lead (Lub Limtiam) |
Kev npaj antigen | 1. Cov neeg siv khoom muab cov antigen2. Bioantibody npaj cov antigen | / |
Kev txhaj tshuaj nas | Kev txhaj tshuaj ntawm BALB/c nas, kev sau cov ntshav thiab ELISA tsom xam | 4 |
Cell fusion thiab tshuaj ntsuam | Fusion ntawm nas splenocytes thiab myeloma hlwb, HAT kev tshuaj ntsuam | 2 |
Stable cell kab tsim | Subcloning ntawm kev tshuaj ntsuam zoo clones | 3 |
Antibody isotype kev txheeb xyuas | Kev txheeb xyuas cov kab xov tooj ntawm tes subtypes | 1 |
Me me incubation | Serum-dawb incubation | 2 |
Loj loj incubation thiab purification | 200mL tsis muaj tshuaj tua kab mob thiab purification | 1 |